The 21-year-old will serve the suspension immediately meaning he will miss Villa's Sky Bet Championship trip to Reading on Tuesday - as well as their Second City derby with Birmingham at the end of the month
Grealish avoided punishment in Saturday's 1-1 draw with Wolves after he stamped on Conor Coady during the game at Villa Park
England Under-21 international Grealish accepted the charge but felt the three-game ban was excessive
His claim was rejected.
An FA statement read: " Aston Villa's Jack Grealish will serve a three-match suspension with immediate effect after he committed an act of violent conduct which was not seen by the match officials but caught on video."
He will also miss Saturday's visit of Fulham in a blow to new boss Steve Bruce who took charge at the club last week
The Wolves draw was his first game in charge and left Villa 20th in the table and one point above the relegation zone
They have won just once this season and Roberto Di Matteo was sacked earlier this month after only 12 games at the club
Source : PA